Soil research: atomic absorption spectrophotometry

Atomic absorption spectrophotometry is an analytical technique based on the selective absorption of electromagnetic radiation by atoms. A sample is atomised in a flame. Light is then beamed into the flame with a lamp. The irradiated light is absorbed by the atoms in the flame. The degree of absorption is determined by the concentration of atoms in the flame and therefore also by the concentration of atoms in the sample. With this technique, metal atoms are measured. 

 

Plant colours extract research: VIS Spectrophotometry

 

UV/VIS spectrophotometry is a spectroscopic technique in which the concentration of a certain substance in a sample to be analysed is determined by measuring the absorption of visible light (VIS) or ultraviolet light (UV).

Using ultraviolet light is referred to as UV spectrometry and using visible light is referred to as visible-light spectrometry, but the technique is basically the same in both cases.

Some chemical compounds have the property of absorbing light of a certain wavelength of the spectrum. The concentration of such a compound in a mixture can be determined by examining the absorbance (also called absorbance) of monochromatic light of the appropriate wavelength.